Using the Crispr technology, the conditional overexpression structure of CAG promoter-loxp-STOP-loxp-Myc-polyA was inserted into the H11 locus to generate an H11-LSL-Myc mouse model. Located on mouse chromosome 11, the H11 locus is similar to Rosa26 and can be used to express a wide range of exogenous genes. The Myc gene can be highly expressed in Cre-expressing tissues after mating the H11-LSL-Myc mice with Cre mice.
For example: After mating H11-LSL-Myc mice with Alb-cre mice, a liver cancer can be spontaneously developed at 2 months old. This mouse model can be used in the establishment of tumor models and tumor research.
Fig1. Schematic diagram of H11-LSL-Myc knockin cassette.
Fig2 Representative in vivo bioluminescence imaging showing the growth of tumors in C57BL/6 mice inoculated with hepatocellular carcinoma tissue from H11-LSL-Myc; Rosa26-LSL-Luc-EGFP; Alb-Cre mice.
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