Model Description
During the PCR of genotyping, genomic DNA is usually extracted from mouse tail tissues by crude extraction. The genomic DNA obtained by this extraction method contains a high amount of impurities, which may affect the efficiency of subsequent PCR. Consider improving the extraction method to ensure the quality of extracted genomic DNA. In the PCR system, the concentration of genomic DNA can also affect the efficiency of PCR. Too much or too little genomic DNA can lead to the failure of PCR amplification. Adjusting the PCR system might be a solution. If PCR shows non-specific bands, consider increasing the annealing temperature to enhance the specific binding of the primers.
