hOSM

Fig.1 Detection of OSM expression from splenocytes by ELISA. Splenocytes were isolated from female 10-week-old wild-type (WT, female, 10 weeks old) and homozygous hOSM knock-in (HO, female, 10 weeks old) mice, then seeded in 12-well plates at a density of 2×106 cells/well in 2 mL culture medium. Following in vitro stimulation with 5 μg/mL anti-mouse CD3 and 5 μg/mL anti-mouse CD28 for 24 or 48 h, human OSM levels in the culture supernatants were measured using a human OSM ELISA kit. Human OSM was detectable exclusively in HO mice. 

Abbr. N.D., not detected.