Model Description
<p style="text-align: justify;">For knockout or genetically mutated mice with an inbred background, mating between heterozygous knockout mice is generally carried out to obtain homozygous knockout or genetically mutated mice. At this time, wild-type or heterozygous mice born in the same litter are generally selected as the control group for the experiment.
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<img src="default/20180404/ce15d4e716103a5b4936f977a6b023d1.jpg" title="FAQ-数据图-CS5-02-xg.jpg" alt="FAQ-数据图-CS5-02-xg.jpg" class="img-responsive">
Figure 2. Breeding procedure for systemic knockout mice.
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<strong>Removal of neo from knockout mice</strong>
<p style="text-align: justify;">If the removal of neo may cause a complete loss of gene functions and if there are no other genes in the vicinity of the genome, the neo gene does not have to be removed. On the other hand, if only a certain functional domain is knocked out, some proteins with partial functions may appear. Alternatively, there may be other genes located around the knockout gene. In these two cases, it is suggested to remove the neo gene to avoid the occurrence of a phenotype not consistent with the gene knockout. If there is sufficient time, it is suggested to remove the neo gene to avoid the occurrence of other phenotypes caused by neo.
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