Using CRISPR/Cas9 technology, gRNAs targeting exon1 of Il7 gene were used to generate Il7 knockout mice. Il33基因位于小鼠9号染色体。通过CRISPR/Cas9途径，设计sgRNA针对Il33基因exon 2，建立该Il33基因敲除小鼠模型。
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